1290 HPLC  

The Agilent 1290 Infinity LC system provides the highest levels of speed, resolution, flexibility and sensitivity for any LC and LC/MS application. 

 

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1290 Infinity Series HPLC

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 Used Agilent 1290 Infinity Series HPLC System · Download Hplc Agilent Technologies1290 Infinity II LC System Brochure and Guide. Downloa

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What Is Evaporative light scattering detector (ELSD)?  

An evaporative light scattering detector (ELSD) is a detector used in conjunction with high-performance liquid chromatography (HPLC). It is commonly used for analysis of compounds that do not absorb UV radiation and therefore cannot be detected by UV detectors, such as sugars, antivirals, antibiotics, lipids, phospholipids, terpenoids, and alcohols.ELSDs fall under the category of general-purpose detectors, similar to refractive index detectors (RI)./wikipedia

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HPLC Column | Agilent MicroBore Small Molecule Separations LC Column  

MicroBore (1 mm id) columns are a good choice when sample sizes are limited. They will improve detection limits 5 times over 2.1 mm id columns when the same sample mass is used. This increase in sensitivity can be critical. MicroBore columns use low flow rates (typically ~50 µL/min). Therefore, these columns are ideal for use with detectors requiring low flow ratessuch as some mass spectrometers. MicroBore columns perform optimally with HPLC systems purchased or modified for microbore use. Guard columns are now available with an adjustable tube stop depth to provide a perfect zero dead volume connection every time.
Features Of Agilent MicroBore Small Molecule Separations LC Column:
High sensitivity for small sample sizes
Compatible with LC/MS interfaces
Wide variety of bonded phases
G

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History Of Chromatography  

Chromatography was first employed in Russia by the Italian-born scientist Mikhail Tsvet in 1900. He continued to work with chromatography in the first decade of the 20th century, primarily for the separation of plant pigments such as chlorophyll,carotenes, and xanthophylls. Since these components have different colors (green, orange, and yellow, respectively) they gave the technique its name. New types of chromatography developed during the 1930s and 1940s made the technique useful for many separation processes.
Chromatography technique developed substantially as a result of the work of Archer John Porter Martin and Richard Laurence Millington Synge during the 1940s and 1950s, for which they won a Nobel prize.They established the principles and basic techniques of partition chromatograph

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Column Chromatography is the the first form of techniques developed in chromatography!  

Column chromatography is the prototype or the basic type of chromatography. It was the first form of techniques developed in chromatography. One can easily explain the principle and procedure of chromatography using it.
Other types of chromatography techniques like high performance liquid chromatography (HPLC), gas chromatography (GC), paper chromatography were developed with column chromatography as a module and making slight variations.
In-spite of many advanced methods of chromatography, still this method of chromatography is widely used in research and industry.
This chromatography is basically a type of adsorption chromatography techniques.

Here the separation of components depends upon the extent of adsorption to stationary phase. Here the stationary phase is a polar solid materia

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High performance liquid chromatography (HPLC)  

High Performance Liquid Chromatography (HPLC) is a form of column chromatography that pumps a sample mixture or analyte in a solvent (known as the mobile phase) at high pressure through a column with chromatographic packing material (stationary phase). The sample is carried by a moving carrier gas stream of helium or nitrogen. HPLC has the ability to separate, and identify compounds that are present in any sample that can be dissolved in a liquid in trace concentrations as low as parts per trillion. Because of this versatility, HPLC is used in a variety of industrial and scientific applications, such as pharmaceutical, environmental, forensics, and chemicals.

Sample retention time will vary depending on the interaction between the stationary phase, the molecules being analyzed, and the s

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Displacement Chromatography  

The basic principle of displacement chromatography is: A molecule with a high affinity for the chromatography matrix (the displacer) will compete effectively for binding sites, and thus displace all molecules with lesser affinities. There are distinct differences between displacement and elution chromatography. In elution mode, substances typically emerge from a column in narrow, Gaussian peaks. Wide separation of peaks, preferably to baseline, is desired in order to achieve maximum purification. The speed at which any component of a mixture travels down the column in elution mode depends on many factors. But for two substances to travel at different speeds, and thereby be resolved, there must be substantial differences in some interaction between the biomolecules and the chromatography m

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Size Exclusion Chromatography ( SEC )  

Size-exclusion chromatography (SEC), also known as gel permeation chromatography or gel filtration chromatography, separates particles on the basis of molecular size (actually by a particle's Stokes radius). It is generally a low resolution chromatography and thus it is often reserved for the final, "polishing" step of the purification. It is also useful for determining the tertiary structure and quaternary structure of purified proteins. SEC is used primarily for the analysis of large molecules such as proteins or polymers. SEC works by trapping these smaller molecules in the pores of a particle. The larger molecules simply pass by the pores as they are too large to enter the pores. Larger molecules therefore flow through the column quicker than smaller molecules, that is, the smaller the

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HPLC UV, VIS, and PDA Detectors  

The UV, VIS, and PDA detectors are categorized as absorbance detectors. They provide good sensitivity for light-absorbing compounds at ~pg level. They are easy to operate and provide good stability. UV detector is a very commonly used detector for HPLC analysis. During the analysis, sample goes through a clear color-less glass cell, called flow cell. When UV light is irradiated on the flow cell, sample absorbs a part of UV light. Thus, the intensity of UV light observed for the mobile phase (without sample) and the eluent containing sample will differ. By measuring this difference, the amount of sample can be determined. Since the UV absorbance also differs depend on what wavelength is used, it is important to choose an appropriate wavelength based on the type of analyte. A standard UV det

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HPLC Column | Agilent LiChrospher Small Molecule Separations LC Column  

Agilent LiChrospher silica columns are made with spherical, 'sil' type porous silica particles. LiChrospher 60 RP-select B starts with 60Å pore size silica optimized for symmetrical peak shapes of basic compounds. LiChrospher 100Å is offered in both C8 and C18 as well as an endcapped C18. These columns are noted for high sample capacity and efficiency. The popular LiChrospher packings are offered in the convenient and economical Agilent cartridge configuration. For normal phase chromatography, Agilent supplies polar modified silica gel phases LiChrospher Si, LiChrospher NH2 , LiChrospher DIOL and LiChrospher CN as convenient cartridge columns.
Features Of Agilent LiChrospher Small Molecule Separations LC Column:
Available in 5 µm particle size
Reversed-phase available in RP-C18, RP-18

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HPLC Column | Agilent ChiraDex Small Molecule Separations LC column  

Applications: Barbiturates, benzothioadiazines, calcium antagonists, PTH-amino acids
Features Of Agilent ChiraDex Small Molecule Separations LC column:
For routine separation of enantiomers
Available as ChiraDex cartridge columns
Novel manufacturing process bonds ß-cyclodextrin to spherical 5-um silica gel by means of a chemical spacer
Enantiomeric separations have been achieved with ChiraDex using simple nonchiral solvent systems

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Stationary Phase In Column Chromatography  

The stationary phase or adsorbent in column chromatography is a solid. The most common stationary phase for column chromatography is silica gel, the next most common being alumina. Cellulose powder has often been used in the past. Also possible are ion exchange chromatography,reversed-phase chromatography (RP),affinity chromatography or expanded bed adsorption (EBA). The stationary phasesare usually finely ground powders or gels and/or are microporous for an increased surface, though in EBA a fluidized bed is used.
There is an important ratio between the stationary phase weight and the dry weight of the analyte mixture that can be applied onto the column. For silica column chromatography, this ratio lies within 20:1 to 100:1, depending on how close to each other the analyte components are

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HPLC Column | Agilent ZORBAX Eclipse Amino Acid Analysis (AAA) LC Column  

The ZORBAX Eclipse AAA high efficiency HPLC column rapidly separates amino acids following an updated and improved protocol for use with the Agilent 1100 HPLC. Total analysis from injection to injection can be achieved in as little as 14 min (10 min analysis time) on the short, 7.5 cm column and 24 min (16 min analysis time) on the 15 cm column length. Exceptional sensitivity (5 - 50 pmol with DAD,FLD) and reliability are achieved using both OPA and FMOC derivitization chemistries in one fully automated procedure using the Agilent 1100 HPLC instrument. Eclipse AAA columns are used for high resolution rapid analysis of 24 amino acids. The Eclipse AAA is use tested for amino acid analysis (AAA). The Eclipse AAA uses well known OPA and FMOC derivatization techniques.
Features Of  Agilent ZOR

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HPLC Column | Agilent ZORBAX Extend-C18 Small Molecule Separations LC Column  

Extend-C18 columns incorporate a unique patented bidentate silane, combined with a double-endcapping process that protects the silica from dissolution at high pH - up to pH 11.5. Columns are best applied for separations of compounds that are either (1) basic and have little or no retention at low or intermediate pHs, (2) more stable or more soluble at high pH, or (3) basic and show poor peak shape at low or intermediate pH.
Features Of Agilent ZORBAX Extend-C18 Small Molecule Separations LC Column:
High efficiency and long life at high pH - up to pH 11.5
Unique bidentate bonding and double endcaping provides high pH stability
More efficiency and better peak shape than polymer-based columns
Improve retention, resolution and peak shape of basic compounds
High sensitivity for LC/MS sepa

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Agilent HPLC 1200  

The Agilent 1200 Series HPLC System was introduced in 2010 with a modular design allowing users to define a configuration ideally suited to meet their HPLC and UHPLC applications and requirements. 

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HPLC Column | Agilent Load & Lock Small Molecule Separations LC Column  

Agilent offers a complete range of Load & Lock column systems for laboratory and process preparative LC. They are designed to enable users to easily and quickly pack their own preparative high efficiency columns. This is the right solution for applications ranging in scale from discovery (milligrams) to production (multi-kilos) of pharmaceutical compounds, peptides, and natural products. Our Load & Lock columns have a unique fluid/sample distribution system to maximize productivity. It is the only system that provides dynamic axial compression (DAC) and static "locked" axial compression (SAC) and is designed for easy operation to deliver greater convenience.
Features Of Agilent Load & Lock Small Molecule Separations LC Column:
Mobile packing station supports three different co

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HPLC Column | Agilent Prep Small Molecule Separations LC Column  

[caption id="" align="aligncenter" width="475"] Agilent Prep Small Molecule Separations LC Column[/caption]
Agilent Prep LC Columns are designed for high loadability to purify milligram to gram quantities of products. Column dimensions are available for most preparative sample types ranging from high throughput samples generated in drug discovery to lab scale prep and up to some pilot scale sample preparation. Preparative sized columns are available in 21.2, 30 and 50 mm internal diameters with lengths ranging from 50–250 mm. Columns are available in 5 and 10 µm particle sizes with very high efficiency in every dimension.
Features Of Agilent Prep Small Molecule Separations LC Column:
High loadability for maximum sample purification
Easy scalability from 4.6 mm id to 50 mm id for rapid

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HPLC Column | Agilent ZORBAX Carbohydrate Analysis Small Molecule Separations LC Column  

ZORBAX Carbohydrate Analysis Columns are reproducible, efficient, and flexible. These columns use ZORBAX porous silica microsphere technology. Silica manufacture, bonding and packing are all performed in our ISO9001 facilities. ZORBAX Carbohydrate Analysis Columns can handle high volume injections as much as 50 µL on a 4.6 x 150 mm column.
Features Of Agilent ZORBAX Carbohydrate Analysis Small Molecule Separations LC Column:
Reproducible
Efficient – uses ZORBAX porous silica microsphere technology; silica manufacturing, bonding and packing are all performed in Agilent's ISO 9001 facilities
Flexible – can handle high volume injections – as much as 50 μL on a 4.6 x 150 mm column
Recommended for use with refractive index detectors (RID)

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HPLC Column | Agilent ZORBAX PrepHT Small Molecule Separations LC Column  

ZORBAX optimized phases are now available in preparative dimensions as PrepHT columns. These columns are 21.2 mm and larger in diameter with 5 µm and 7 µm particles to provide high resolving power. They are also available in various lengths to allow optimum resolving power and speed of analyses. In preparative chromatography, it is important to obtain high purity and high recovery along with high throughput. Agilent ZORBAX PrepHT columns have a broad range of selectivities to achieve these objectives.
Features Of Agilent ZORBAX PrepHT Small Molecule Separations LC Column:
Easy scale-up from analytical to preparative scale with ZORBAX phases
Fast preparative separations, up to 2000 mg
5 to 7 μm particles for high efficiency and high yield
Easy to install finger-tight connections seal

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Evaporative Light Scattering Detector (ELSD)  

An evaporative light scattering detector (ELSD) is a detector used in conjunction with high-performance liquid chromatography (HPLC). It is commonly used for analysis of compounds where UV detection might be a restriction and therefore compounds does not very efficient absorb UV radiation, such as sugars, antivirals, antibiotics, lipids, phospholipids, terpenoids, and alcohols. ELSDs fall under the category of general-purpose detectors, similar to refractive index detectors (RI).

ELSD | Evaporative Light Scattering Detector

elsd.hplchplc.com/

 

... Scattering Detector. An evaporative light scattering detector (ELSD) is a detector used in conjunction with high-performance liquid chromatography (HPLC).

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P

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Agilent HPLC 1260  

The Agilent 1260 Infinity HPLC-Chip/MS system is a re-useable microfluidic chip-based technology for high sensitivity nanospray LC/MS. The Agilent 1260 replaced the Agilent 1200 and delivers results faster to save time and money. 

Agilent Technologies 1260 Series

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This Agilent 1260 HPLC included: Binary pump with switch valve 1260 Binary Pump Thermostatted Column Compartment (TCC SL+)

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HPLC Chemiluminescent Nitrogen Detector (CLND)  

Similar to FL, but instead of using a light source to excite the analyte atoms, the excitation is initiated by chemical reaction. Since it is not relied on the external excitation source, the noise is small, results in high signal to noise ratio, i.e. it provides even higher sensitivity than FL.
The Chemiluminescent Nitrogen Detector (CLND) for use with high-performance liquid chromatography (HPLC) allows for the low-level detection of nitrogen-containing compounds with simple quantitation. The nitrogen selective detector’s equimolar response (i.e., equal response for nitrogen independent of its chemical environment) allows for any nitrogen-containing compound to be quantitated as long as the number of nitrogens are known. The HPLC–CLND provides a new detection method for analy

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Principles Of Operation In Evaporative light Scattering Detector (ELSD)  

ELSDs analyze solvent after elution from HPLC. As the eluent passes from an HPLC, it is mixed with an inert carrier gas and forced through a nebulizer, which separates the liquid into minute aerosolized droplets. These droplets then pass into a heated drift tube, where the mobile phase solvent is evaporated off. As the mobile phase evaporates, the droplets become smaller and smaller until all that is left is minute particles of dried analyte. These particles are pushed through the drift tube by the carrier gas to the detection region. In this region, a beam of light crosses the column of analyte and the scattering of light is measured by a photo multiplier tube./wikipedia

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HPLC Refractive-Index (RI) Detector  

RI detector measures change in reflex index. A glass cell is divided into two chambers (cells). The effluent from LC column flow through the "sample cell", while other cell called "reference cell" is filled with only mobile phase. When the effluent going through the sample cell does not contain any analyte, the solvent inside both cells are the same (Figure 1A). When a beam is irradiate on the cells, the observed beam will be straight in this case. However, in a case the effluent contains any components other than mobile phase; bending of the incident beam occurs due to the reflex index difference between the two solvents (Figure 1B). By measuring this change, the presence of components can be observed.

RI detector has lower sensitivity compared to UV detector, and that's the main reason

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1100 HPLC  

Introduced in 1995, Agilent's 1100 series HPLC system is considered the world's most popular HPLC. They are incredibly robust machines that can last a long time. Finding a used Agilent 1100 on LabX is easy and maintenance for older machines consists of changing seals, filter solvents, lamps, and the odd part in the vacuum degasser. Parts from the Agilent 1050 are often shared with the Agilent 1100 which make it easy to maintain.

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Agilent Technologies 1100 Series

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Agilent Technologies is an American public research  

Agilent Technologies is an American public research, development and manufacturing company established in 1999 as a spin-off from Hewlett-Packard. The resulting IPO of Agilent stock was the largest in the history of Silicon Valley at the time.
The company provides analytical instruments, software, services and consumables for the entire laboratory workflow. Agilent focuses its products and services on six markets: food, environmental and forensics, pharmaceutical, diagnostics, chemical and energy, and research. From 1999 to 2014 the company also produced test and measurement equipment, this division was spun out to form Keysight.
Based on 2003 information, Agilent maintained four locations in the San Francisco Bay area: San Jose, Santa Clara, Santa Rosa and Rohnert Park.Santa Clara is an

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Shimadzu Refractive Index LC Detector 20A (RID-20A)  

Inheriting the stability and extensibility that are the strengths of the Prominence series, the new Shimadzu Refractive Index LC Detector 20A (RID-20A) model of differential refractive index detector is designed with a new reference-cell auto-purge feature and validation support function.

Specifications :

 
Pressure Relief Valve :
The RID-20A incorporates various safety features. Its maximum pressure is five times that of former Shimadzu products and, as a standard feature, it incorporates a sensor that detects leakage from the cell unit. For extra safety, a pressure relief valve that prevents problems related to back-pressure irregularities is also available as an option.

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Ion Exchange Chromatography / Ion chromatography  

In ion-exchange chromatography (IC), retention is based on the attraction between solute ions and charged sites bound to the stationary phase. Solute ions of the same charge as the charged sites on the column are excluded from binding, while solute ions of the opposite charge of the charged sites of the column are retained on the column. Solute ions that are retained on the column can be eluted from the column by changing the solvent conditions (e.g. increasing the ion effect of the solvent system by increasing the salt concentration of the solution, increasing the column temperature, changing the pH of the solvent, etc...).
Types of ion exchangers include:

Polystyrene resins – These allow cross linkage which increases the stability of the chain. Higher cross linkage reduces swerving,

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What Is Column chromatography?  

For more details on this topic, see Column chromatography.
Column chromatography is a separation technique in which the stationary bed is within a tube. The particles of the solid stationary phase or the support coated with a liquid stationary phase may fill the whole inside volume of the tube (packed column) or be concentrated on or along the inside tube wall leaving an open, unrestricted path for the mobile phase in the middle part of the tube (open tubular column). Differences in rates of movement through the medium are calculated to different retention times of the sample.
In 1978, W. Clark Still introduced a modified version of column chromatography called flash column chromatography (flash).The technique is very similar to the traditional column chromatography, except for that the

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HPLC Column | Agilent HC-C18(2) Small Molecule Separations LC Column  

Agilent HC-C18(2) columns are an excellent choice for separations of many types of compounds.
Agilent HC-C18(2) is a more retentive C18 with a higher carbon load. An excellent value alternative to other high carbon load columns, it also provides superior peak shape for basic compounds.
Features Of Agilent HC-C18(2) Small Molecule Separations LC Column:
Higher carbon load - 17% - provides greater retention for moderately polar and non-polar compounds
Ideal for non-polar compounds and separations that start at mid-level % organic (at least greater than 10% organic)
Good choice for industrial samples or samples dissolved in organic/mostly organic solvents
Stable over a very wide pH range (2-9) for maximum flexibility
To compare technical information and specifications for different pha

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Agilent HPLC  

Find great deals on HPLCHPLC.COM for Used and Refurbished  Agilent HPLC in Analytical Lab Instruments. Shop with confidence. 





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agilent 1200 hplc


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This HP/Agilent 1200 Series Nano HPLC system has just been moved from a working lab. It comes with the followings: G1379B Degasser with Tray G2226A ...
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Agilent Technologies 1260 Series


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HPLC (high performance liquid chromatograph ) Instrumentation  

Main components in an HPLC system include the solvent reservoir, or multiple reservoirs, a high-pressure pump, a column, injector system and the detector.

The reservoir holds the solvent, which is referred to as the mobile phase because it moves. There are usually a minimum of two reservoirs in a system, with each holding up to 1000 cc of solvent and usually fitted with a gas diffuser through which helium can be bubbled. A pump is used to generate a specified flow of the mobile phase. Although manual injection of samples is still possible, most HPLCs are now fully automated and controlled by computer. The injector, or auto sampler, introduces the solvent into a phase stream that carries the sample into the high pressure (up to 400 bar) column, which contains specific packing material nee

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Normal phase Chromatography  

Normal–phase chromatography was one of the first kinds of HPLC that chemists developed. Also known as normal-phase HPLC (NP-HPLC) this method separates analytes based on their affinity for a polar stationary surface such as silica, hence it is based on analyte ability to engage in polar interactions (such as hydrogen-bonding or dipole-dipole type of interactions) with the sorbent surface. NP-HPLC uses a non-polar, non-aqueous mobile phase (e.g. Chloroform), and works effectively for separating analytes readily soluble in non-polar solvents. The analyte associates with and is retained by the polar stationary phase. Adsorption strengths increase with increased analyte polarity. The interaction strength depends not only on the functional groups present in the structure of the analyte molecu

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chromatography  

Chromatography is a physical method of separation that distributes components to separate between two phases, one stationary (stationary phase), the other (the mobile phase) moving in a definite direction. The eluate is the mobile phase leaving the column. The eluent is the solvent that carries the analyte.

Chromatography

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Chromatography is a physical method of separation that distributes components to separate between two phases, one stationary (stationary phase), the other (the mobile phase) moving in a definite direction. The eluate is the mobile phase leaving the column. The eluent is the solvent that carries the analyte.


Partition Chromatography


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Partition chromatography was one of the first k

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waters hplc  

Whatever your application, Alliance® HPLC, BreezeTM 2 HPLC, and AutoPurificationTM systems offer you proven solutions for all of your HPLC requirements - for today and the future. These systems are synonymous with dependable, routine performance and versatility.

Alliance HPLC Providing versatile, dependable performance for analytical labs, the Alliance HPLC family meets the rigorous requirements of routine chromatography and the performance standards of new-product research and method development.

Breeze 2 HPLC Comes with a pump, detector, injector, and is pre-configured for different levels of HPLC. It includes Breeze Software, an intuitive interface that is easy to set-up, learn, and use.
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Conquer Scientific Company  

Fatih Büyüksönmez , CEO of Conquer Scientific: Conquer Scientific, we are simply dedicated to supporting researchers with high-quality, pre-owned instruments at the lowest possible cost. While we specialize in ion, gas, and liquid chromatographs, mass spectrometers, and microplate readers, we offer a wide range of laboratory instruments in all other fields. At Conquer Scientific, we strive to provide our customers with excellent service and support, whether it is assisting with setup, repairing instruments, or simply providing guidance through a smooth ordering process. It is our goal to become a one-stop resource for the research community for all your laboratory instrument needs.
Conquer Scientific was established in 2007. Since then, it has grown exponentially, owing to our understa

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